rna  (ATCC)

Journal: bioRxiv

Article Title: Resolving in vitro heterogeneity of host functional responses to HCoV-229E via single-cell analyses

doi: 10.64898/2026.04.17.719293

Figure Lengend Snippet: A) Experimental protocol with sample collection at 24 and 48 hours post infection (hpi) for two duplicate experiments (A and B). For scRNA-seq, this resulted in transcriptomes from 13,913 cells across all samples, including 4,192 mock-infected and 2,724 virus-infected cells at 24 hpi and 3,593 mock-infected and 3,404 virus-infected cells at 48 hpi. B) The viral load across samples after quality control. The median log1p(viral reads) detected in 24 hpi sample A is 7.0 (SD 1.0), in 24 hpi sample B is 7.1 (SD 0.9), and across samples A and B is 7.1 (SD 1.0). The median log1p(viral reads) detected in 48 hpi sample A is 6.6 (SD 1.0), in 48 hpi sample B is 6.4 (SD 1.0), and across samples A and B is 6.5 (SD 1.0). C) Venn diagram representing the number of differentially expressed genes in a cell-level, time-matched 229E-infected versus mock-control differential expression test at a threshold of adjusted p-value < 0.001 and |log ₂ FC| > 2.

Article Snippet: Human coronavirus strain 229E (HCoV-229E) from ATCC ((#VR-740, Lot #70053995) was used for in-house virus batch propagation.

Techniques: Infection, Virus, Control, Quantitative Proteomics

Journal: bioRxiv

Article Title: Resolving in vitro heterogeneity of host functional responses to HCoV-229E via single-cell analyses

doi: 10.64898/2026.04.17.719293

Figure Lengend Snippet: A) The median aggregated accessibility profiles centered on the transcription start sites (TSS; ±3kb) for mock and 229E-infected samples at 24 and 48 hpi. The bold lines show median and mean profiles computed over mean profiles across all samples, while lower opacity profiles show variability across replicates. B) The mean aggregated accessibility profiles centered on the transcription start sites (TSS; ±3kb) for mock and 229E-infected samples at 24 and 48 hpi. The bold lines show median and mean profiles computed over mean profiles across all samples, while lower opacity profiles show variability across replicates. C) Principal component analysis (PCA) of accessibility profiles separates mock-control and 229E infected samples at 24 and 48 hpi. Axes indicate the variance explained by each principal component, marker shapes indicate time point, and marker colors indicate infection status. D) Volcano plots of differential accessibility (infected versus mock-control) at 24 (left) and 48 (right) hpi. Each point is an open chromatin region, and blue points indicate significantly increased accessibility, while orange points indicate significantly decreased accessibility. Gray points are not significant. At 24 hpi, 6% of open chromatin regions (OCRs) show increased chromatin accessibility (blue) and 5% show decreased chromatin accessibility (orange). At 48 hpi, 20% of OCRs show increased chromatin accessibility (blue), and 13% show decreased chromatin accessibility (orange).

Article Snippet: Human coronavirus strain 229E (HCoV-229E) from ATCC ((#VR-740, Lot #70053995) was used for in-house virus batch propagation.

Techniques: Infection, Control, Marker